Optics, Photonics and Biophotonics Lab

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From left to right: Luca Tramarin, Beatriz Santamaria, Sergio Quintero, Ana López, Rafael Casquel, Alvaron Lavín, Maria Jesús Pioz, Miguel Holgado, Victor Canalejas, Marife Laguna, Pedro Herreros, Santiago Velasco, Rocio López, Yolanda Ramírez and Javier Sanza

The optics, photonics and biophotonics group is composed of 6 doctors, 7 PhD  candidates and other students developing their bachelor´s or master´s thesis.  Among the doctors of the group, 4 are professors at the School of Industrial Engineering (ETSII) in the department of applied physics and material Engineering and the rest of them are in their post-doctoral stage.

Our work includes all the value chain, since basic investigation until the development of prototypes. Most of them are focused on the detection of proteins, virus and important biomarkers without the necessity of chemical development, e.  g. Biochip-KIT. Also, we develop a reading method for the biochips, using optical read-out systems very useful for in vitro detection.

Recently, in collaboration with other groups, we are developing systems to immobilize cells with the goal of replace the experimentation in animal systems.

Our group projects are focus in basic investigation and their application in the industrial development with funding of national and international agencies and companies.

Moreover, we participate in projects of science outreach, to promote science vocations in the younger students.

Finally, our group created in 2010 the company BIOD, whose implement and commercialize some the technology developed by the group.

WHO WE ARE

HR March 2019-2

CONTACT INFORMATION

Group leader: Dr. Miguel Holgado
Phone number: 91 336 46 36 / 38
Mail:  m.holgado@upm.es

Principal Research lines

Photonic transducers for label-free biosensors.
Optical read-out platforms for the development of Point-of-care devices  to present information of clinical interest.
Packaging and KITs focused on multiplexation and user-friendly
Micro-nano fluidics for lab-on-a-chip systems.
Fluidic chips for cells-on-a-chip systems.
Surface functionalization  for bio-receptors and chemical applications.

INFRASTRUCTURE AND TECHNIQUES

The Biophotonics Laboratory is under clean conditions and temperature control, and it is also equipped with clean dry and dust-less air

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MICRO-NANO FABRICATION

• Processing protocols for wet etching and cleaning
• Thin films deposition by spin-coating up to 150 mm wafers,
• Laminar flow class 10 chamber
• Thin films Chemical Vapor deposition CVD (self-produced ) for SiO2 and TiO2
• Thermal processes by hot plates
• Precision Balances
• UV projection lithography mask (resolution up to 500 nm) up to 150 mm wafers
• Nano-imprint lithography (UV-NIL)
• Manufacture by stamping and mold for polymers
• Developing processes for micro-nano lithography
• Fast prototyping manufacture by 3D printer

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OPTICAL CHARACTERIZATION AND READ-OUT SYSTEMS

• Read-out system for continuous flow biomolecular interaction system
• Point of Care systems manufactured by BIOD and designed by the Research group.
• Optical-fiber thin-film metrology (1 nm resolution) UV-VIS for measuring thin film thickness and equipped with camera capable of measuring up to 0.2 mm domains
• FT-VIS-NIR high-resolution spectrometry (pm resolution) and IR spectroscopy in transmission and reflection for VIS-NIR capable of measuring domains at the micrometer domains, also equipped with a microscope.
• Sub-micrometric spatial resolution (up to 600 nm) Reflectometry angle -resolved and ellipsometry valid for thickness measurements on thin films and nanostructures response
analysis
• Optical Microscopy

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BIO-FUNCTIONALIZATION: INMOBILIZATION AND OPTICAL IN-VITRO DETECTION OF BIOMOLECULES

• Assays models: IgG annti-IgG / BSA-anti_BSA
• Infectious diseases: Rotavirus, Dengue, Influenza
• Tear-dry eye monitoring: Tear Biomarkers
• Pathogen detection: Bacteria
• Detection of Allergens and Immunological tolerances

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JOURNALS PAPERS IN JCR

AñoTítuloAutoresRevistaDOI
2018How the surrounding environment affects the biosensing performance of resonant nanopillars arrays: Under dry conditions or immersed in fluidAna L. Hernández, Rafael Casquel, Miguel Holgado, Iñaki Cornago, Fátima Fernández, Paula Ciaurriz, Francisco J. Sanza, Beatriz Santamaría, María V. Maigler, Sergio Quintero, María Fe LagunaSensors and Actuators B: Chemicalhttps://doi.org/10.1016/j.snb.2017.11.029
2018Fabrication of Si3N4/SiO2 tiered resonant nanopillars with nickel caps arrays: application for optochemical sensingV. Canalejas-Tejero, A. L. Hernández, R. Casquel, S. A. Quintero, M. F. Laguna, M. HolgadoOptical Material Expresshttps://doi.org/10.1364/OME.8.001082
2018On the determination of uncertainty and limit of detection in label-free biosensorsÁlvaro Lavín, Jesús de Vicente, Miguel Holgado, María F. Laguna, Rafael Casquel, Beatriz Santamaría, María Victoria Maigler, Ana L. Hernández, Yolanda RamírezSensorshttps://doi.org/10.3390/s18072038
2018A Proof-of-Concept of Label-Free Biosensing System for Food Allergy Diagnostics in Biophotonic Sensing Cells: Performance Comparison with ImmunoCAPRocio L. Espinosa, María Fe Laguna, Fátima Fernández, Beatriz Santamaria, Francisco Javier Sanza, Maria Victoria Maigler, Juan J. Álvarez-Millán, Víctor Canalejas-Tejero, Miguel HolgadoSensorshttps://doi.org/10.3390/s18082686
2017Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ DiagnosisBeatriz Santamaría, María F. Laguna, David López-Romero, Ana L. Hernandez, Francisco J. Sanza, Álvaro Lavín, Rafael Casquel, María V. Maigler, Rocío L. Espinosa, Miguel HolgadoSensorshttps://doi.org/10.3390/s17051158
2017Micron-scale wedge thin films prepared by plasma enhanced chemical vapor depositionMaria-Carmen López-Santos, Rafael Alvarez, Alberto Palmero, Ana Borrás, Rafael Casquel del Campo, Miguel Holgado, Agustín R. González-ElipePlasma Processes and Polymershttps://doi.org/10.1002/ppap.201700043
2017Optical sensor based on periodic array of resonant nanopillars for real time monitoringFátima Fernández, Paula Ciaurriza, Iñaki Cornago, R. Casquel, Ana L. Hernandez, F.J. Sanza, M.F. Laguna, M. HolgadoSensors and Actuators B: Chemicalhttps://doi.org/10.1016/j.snb.2016.12.140
2016Dye-based photonic sensing systemsFrancisco J. Aparacio, María Alcaire, Agustín R. González-Elipe, Ángel Barranco, Miguel Holgado, Rafael Casquel, Francisco J. Sanza, Amadeu Griol, Damien Bernier, Santiago Cáceres, Mikael Antelius,Martin Lapisa, Hans Sohlström,Frank NiklausSensors and Actuators B: Chemicalhttp://dx.doi.org/10.1016/j.snb.2016.01.092
2016Sensitive metal layer-assisted guided-mode resonance SU8 nanopillar array for label-free optical biosensingVíctor Canalejas-Tejero, Ana López, Rafael Casquel, MiguelHolgado, Carlos Angulo BarriosSensors and Actuactors B: Chemicalhttp://dx.doi.org/10.1016/j.snb.2015.11.114
2016Towards reliable optical label-free point-of-care (PoC) biosensing devicesM. Holgado, M. V. Maigler, B. Santamaría, A.L. Hernández, A. Lavín, M.F. Laguna, F.J. Sanza, D. Granados, R. Casquel, J. Portilla, T. RiesgoSensors and Actuators B: Chemicalhttp://dx.doi.org/10.1016/j.snb.2016.06.047
2016Bulk sensing performance comparison between silicon dioxide and resonant high aspect ratio nanopillars arrays fabricated by means of interference lithographyI. Cornago, A. L. Hernández, R. Casquel, M. Holgado, M. F. Laguna, F. J. Sanza, J. BravoOptical Material Expresshttps://doi.org/10.1364/OME.6.002264
2015Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA TechniqueMaríafe Laguna, Miguel Holgado, Ana L. Hernandez, Beatriz Santamaría, Alvaro Lavín, Javier Soria, Tatiana Suarez, Carlota Bardina, Mónica Jara, Francisco J. Sanza, Rafael CasquelSensorshttps://doi.org/10.3390/s150819819
2015Arrays of resonant nanopillars for biochemical sensingAna L. Hernández, R. Casquel, M. Holgado, I. Cornago, F. J. Sanza, B. Santamaría, M. Maigler, F. Fernández, A. Lavín, M. F. LagunaOptics Lettershttps://doi.org/10.1364/OL.40.002370
2015Sub-micrometric reflectometry for localized label-free biosensingR. Casquel, J.A. Soler, M. Holgado, A. López, A. Lavín, J. de Vicente, F.J. Sanza, M.F. Laguna, M.J. Bañuls, R. PuchadesOptics Expresshttps://doi.org/10.1364/OE.23.012544
2014Label-free biosensing by means of BICELLs for dry eyeMaría Fe Laguna, Francisco J. Sanza, J. Soria, Mónica Jara, Álvaro Lavín, Rafael Casquel, A. López, T. Suarez, Miguel HolgadoSensors and Actuactors B: Chemicalhttp://dx.doi.org/10.1016/j.snb.2014.06.114
2014Optimization of dengue immunoassay by label-free interferometric optical detection methodMaría F. Laguna, Miguel Holgado, Francisco J. Sanza, Alvaro Lavín, Ana López, Rafael CasquelSensorshttps://doi.org/10.3390/s140406695
2014Silicon nanopillar arrays with SiO2 overlayer for biosensing applicationB. Dev. Choudhury, R. Casquel, M.J. Bañuls, F.J. Sanza, M.F. Laguna, M. Holgado, R. Puchades, A. Maquieira, C.A. Barrios, S. AnandOptical Material Expresshttps://doi.org/10.1364/OME.4.001345
2014Description of an advantageous optical label-free biosensing interferometric read-out method to measure biological speciesMiguel Holgado, Francisco J. Sanza, Ana López 1, Álvaro Lavín 1,2, Rafael Casquel 1,2 and María F. LagunaSensorshttps://doi.org/10.3390/s140203675
2013Efficient design and optimization of bio-photonic sensing cells (BICELLs) for label free biosensingÁlvaro Lavín, Rafael Casquel, Francisco J. Sanza, María Fé Laguna, Miguel HolgadoSensors and Actuators, B: Chemicalhttp://dx.doi.org/10.1016/j.snb.2012.09.058
2013Development of a versatile biotinylated material based on SU-8Francisco J. Ortega, María José Bañuls, Francisco J. Sanza, María F. Laguna, Miguel Holgado, Rafael Casquel, Carlos A. Barrios, David López-Romero, Ángel Maqueira, Rosa PuchadesJournal of Materials Chemistry Bhttps://doi.org/10.1039/c3tb20323a
2012Biomolecular interaction analysis of gestrinone-anti-gestrinone using arrays of high aspect ratio SU-8 nanopillarsFrancisco J. Ortega, María-José Bañuls, Francisco J. Sanza, Rafael Casquel, María Fe Laguna, Miguel Holgado, David López-Romero, Carlos A. Barrios, Ángel Maquieira, Rosa Puchades Biosensorshttps://doi.org/10.3390/bios2030291
2011Bio-Photonic Sensing Cells over transparent substrates for anti-gestrinone antibodies biosensingF.J. Sanza, M. Holgado, F.J. Ortega, R. Casquel, D. López-Romero, M.J. Bañuls, M.F. Laguna, C.A. Barrios, R. Puchades, A. MaqueiraBiosensors and Bioelectronicshttps://doi.org/10.1016/j.bios.2011.06.010
2011UV laser-induced high resolution cleaving of Si wafers for micro-nano devices and polymeric waveguide characterizationR. Casquel, M. Holgado, J.J. García-Ballesteros, K. Zinoviev, C. Férnandez-Sánchez,
F.J. Sanza, C. Molpeceres, M.F. Laguna, A. Llobera, J.L. Ocaña, C. Domínguez
Applied Surface Sciencehttps://doi.org/10.1016/j.apsusc.2010.11.021
2011Cost-effective SU-8 micro-structures by DUV excimer laser lithography for label-free biosensingF.J. Sanza, M.F. Laguna, R. Casquel, M. Holgado, C.A. Barrios, F.J. Ortega, D. López-Romero, J.J. García-Ballesteros, M.J. Bañuls, A. Maquieira, R. PuchadesApplied Surface Sciencehttps://doi.org/10.1016/j.apsusc.2010.10.010
2010High aspect-ratio SU-8 resist nano-pillar lattice by e-beam direct writing and its application for liquid trappingD. López-Romero, C.A. Barrios, M. Holgado, M.F. Laguna, R. CasquelMicroelectronic Engineeringhttp://dx.doi.org/10.1016/j.mee.2009.09.007
2010Label-free biosensing by means of periodic lattices of high aspect ratio SU-8 nano-pillarsM. Holgadoa, C.A. Barrios, F.J. Ortega, F.J. Sanza, R. Casquel, M.F. Laguna,
M.J. Bañuls, D. López-Romero, R. Puchades, A. Maquieira
Biosensors and Bioelectronicshttps://doi.org/10.1016/j.bios.2010.04.042
2010Analysis of plasma thermal surface effects on the residual stress field induced by LSP in Al2024-t351M. Morales, J.A. Porro, C. Molpeceres, M. Holgado, J.L. OcañaJournal of Optoelectronics and Advanced Materialshttps://joam.inoe.ro/index.php?option=magazine&op=view&idu=2395&catid=48
2010Study of the refractive index change in a-Si:H thin films patterned by 532 nm laser radiation for photovoltaic applicationsM. Colina, C. Molpeceres, M. Holgado, J. Gandia, O. Nos, J.L. OcañaThin Solid Filmshttps://doi.org/10.1016/j.tsf.2010.04.016
2010Transparent Nanometric Organic Luminescent Films as UV-Active Components in Photonic StructuresFrancisco J. Aparicio , Miguel Holgado , Ana Borras , Iwona Blaszczyk-Lezak , Amadeu Griol , Carlos A. Barrios , Rafael Casquel , Francisco J. Sanza , Hans Sohlström , Mikael Antelius , Agustin R. González-Elipe , and Angel BarrancoAdvanced Materialshttps://doi.org/10.1002/adma.201003088
2008Label-free optical biosensing with slot-waveguidesCarlos A. Barrios, María José Bañuls, Victoria González-Pedro, Kristinn B. Gylfason, Benito Sánchez, Amadeu Griol, A. Maquieira, H. Sohlström, M. Holgado, R. CasquelOpticsLettershttps://doi.org/10.1364/OL.33.000708
2008Reconfiguration of microring resonators by liquid adhesionC. A. Barrios, M. Holgado, O. Guarneros, K. B. Gylfason, B. Sánchez, R. Casquel, and H. SohlströmApplied Physics Lettershttps://doi.org/10.1063/1.3033528
2008Simultaneous reflectivity, ellipsometry and spectrometry measurements in submicron structures for Liquid sensingM. Holgado, R. Casquel, C. Molpeceres, M. Morales, J.L. OcañaSensor Lettershttps://doi.org/10.1166/sl.2008.430
2007Slot-waveguide biochemical sensorCarlos A. Barrios, Kristinn B. Gylfason, Benito Sánchez, Amadeu Griol, H. Sohlström, M. Holgado, R. CasquelOptics Lettershttps://doi.org/10.1364/OL.32.003080
2007Demonstration of slot-waveguide structures on silicon nitride / silicon oxide platformC. A. Barrios, B. Sánchez, K. B. Gylfason, A. Griol, H. Sohlström, M. Holgado, R. CasquelOptics Expresshttps://doi.org/10.1364/OE.15.006846
2007Optical characterization of extremely small volumes of liquid in sub-micro-holes by simultaneous reflectivity, ellipsometry and spectrometryM. Holgado, R. Casquel, M. Morales, C. Molpeceres, J.L. OcañaOptics Expresshttps://doi.org/10.1364/OE.15.013318

CONFERENCES

AñoTítuloAutoresRevistaDOI
2018Development towards compact nitrocellulose interferometric biochips for dry eye diagnosis based on MMP9, S100A6 and CST4 biomarkers using a Point-of-Care deviceBeatriz Santamaría, María Fe Laguna, David López-Romero, A. López-Hernandez, F. J. Sanza, A. Lavín, R. Casquel, M. Maigler, M. HolgadoOphthalmic Technologies XXVIIIhttps://doi.org/10.1117/12.2288257
2018Design Space Exploration for PCA Implementation of Embedded Learning in FPGAsRodrigo Marino, Jose M. Lanza-Gutierrez, Teresa Riesgo, Miguel Holgado2018 IEEE International Symposium on Circuits and Systems (ISCAS)https://doi.org/10.1109/ISCAS.2018.8351540
2016Resonant nanopillars as label-free optical biosensorsAna L. Hernández, Rafael Casquel, Miguel Holgado, Iñaki Cornago, Fátima FernándezSPIE 2018 Progress in Biomedical Optics and Imaging – Proceedings of SPIEhttps://doi.org/10.1117/12.2288273
2015Direct Laser Interference Patterning (DLIP) technique applied to the development of optical biosensors based on Biophotonic Sensing Cells (BICELLs)F.J. Sanza, D. Langheinrich, J. Berger, A.L. Hernandez, S. Dani, R. Casquel, A. Lavín, A. Otón, B. Santamaría, M. Laguna, A.F. Lasagni, M. HolgadoProceedings of SPIE – The International Society for Optical Engineeringhttps://doi.org/10.1117/12.2077292
2015Performance evaluation for different sensing surface of BICELLs bio-transducers for dry eye biomarkersM.F. Laguna, M. Holgado, B. Santamaría, A. López, M. Maigler, A. Lavin, J. de Vicente, J. Soria, T. Suarez, C. Bardina, M. Jara, F.J. Sanza, R. Casquel, A. Oton, T. RiesgoProgress in Biomedical Optics and Imaging – Proceedings of SPIEhttps://doi.org/10.1117/12.2077174
2014Uncertainty in optical bio-sensors due to the spectral displacement of the interference modes of the transduction signalJ. de Vicente, M. Holgado, A. Lavín, F. J. Sanza, R. Casquel, M. F. LagunaÓptica Pura y Aplicadahttps://doi.org/10.7149/OPA.47.1.27
2008Label-free optical biosensor on a single chipMiguel Holgado, Rafael Casquel,María-Fe LagunasSPIE Newsroom 2008https://doi.org/10.1117/2.1200812.1381
2008Vertical resonant microcativites based on pillars analyzed by beam profile ellipsometry and reflectometryR. Casquel, M. Holgado, A. Lavín, C. A. Barrios, C. Molpeceres, M. Morales, J.L. Ocaña.XXII Eurosensorshttp://oa.upm.es/3384/
2007Photonic sensors based on integrated reflectivity, ellipsometry and spectrometry measurements in submicron size geometriesR. Casquel, M. Holgado, C. Molpeceres, M. Morales, J. L. Ocafia2007 Spanish Conference on Electron Deviceshttps://doi.org/10.1109/SCED.2007.384062

RESEARCH PROJECTS

EUROPEOS

1.1.1.1 Point-of-care device based on KETs for diagnosis of food allergies (ALLERSCREENING)

Entidad Financiadora: Comisión Europea

Referencia CE: H2020-2017-768641

Tipo de Convocatoria: H2020-NMBP-2016-2017/H2020-NMBP-X-KET-2017

Coordinador: Universidad Politécnica de Madrid (UPM)

Entidades participantes: Bio Optical Detection, Consulting Químico Sanitario, Microfluidic ChipShop, Clinica San Carlo, Technische Universität München, Medicinische Universität WIEN, Consejería de Salud de Andalucía, Servicio Andaluz de Salud.

Duración: 48 meses (1 October 2017 a 31 septembre de 2021)

Cuantía de subvención: 1.182.250 € para la UPM (591.125 € para mi grupo de investigación)

Tipo de participación: Co-Investigador Principal de la UPM

1.1.1.2 Development Of A Biosensor Technology For Environmental Monitoring And Disease Prevention In Aquaculture Ensuring Food Safety (ENVIGUARD)

Entidad Financiadora: Comisión Europea

Referencia CE: FP7-OCEAN-2013- 614057

Tipo de Convocatoria: CP-VII Programa Marco de la Unión Europea

Coordinador: TTZ Bremerhaven

Entidades participantes: The Alfred Wegener Institute, The Helmholtz-Zentrum Geesthacht, iSiTEC, CEMITEC, Universidad Politécnica de Madrid (UPM), Bio Optical Detection, Multitel, Biotem, Lionix, The University of Hertfordshire, The AquaBioTech Group, Northbay Shellfish Ltd, Istanbul University, Ilknak Viking Fish Farms, Applied Enzyme Technology, Bioazul

Duración: 50 meses (Octubre de 2013 hasta 1 del 12 de 2018)

Cuantía de subvención: 298.260 € para la UPM

Tipo de participación: Investigador Principal de la UPM

 

NACIONALES

1.1.1.1 Transductores avanzados, biochips y plataformas de lectura para biosensores de alto rendimiento (HERON)

Entidad Financiadora: Ministerio de Economía y Competitividad (MINECO)

Referencia: TEC2017-84846-R

Tipo de Convocatoria: Plan Nacional de I+D. Retos de la Sociedad

Entidades participantes: Universidad Politéncnica de Madrid, más investigadores de: Universidad Politécnica de Valencia, Universidad de la Laguna, Consejo Superior de Investigaciones Científicas

Coordinador: Universidad Poltécnica de Madrid

Duración: 3 años

Cuantía de subvención: 202.000 € en Resolución provisional

Tipo de participación: Investigador Principal

1.1.1.2 Biochip kits basados en celdas biofotonicas y plataformas avanzadas de interrogacion óptica (PLATON)

Entidad Financiadora: Ministerio de Economía y Competitividad (MINECO)

Referencia: TEC2012-31145

Tipo de Convocatoria: Plan Nacional de I+D. Investigación Fundamental no orientada

Entidades participantes: Universidad Politécnica de Madrid, mas investigadores de la Universidad Politécnica de valencia

Coordinador: Universidad Politécnica de Madrid

Duración: De febrero de 2013 a diciembre de 2016

Cuantía de subvención: 134.000 €

Tipo de participación: Investigador Principal

INDUSTRIALES

1.1.1.1 REMO (Monitoreo en tiempo real de propiedades de líquidos mediante transductores fotónicos de funcionalidad específica en sistema de fibra óptica)

Programa competitivo: INSPIRE

Ente Financiadora: REPSOL S.A. e INDRA S.A.

Participantes: Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Madrid

Duración: Septiembre de 2015 con duración de 3 años.

Cuantía de Subvención: 683.343 € (478.215 € para la UPM)

Tipo de participación: Investigador Principal

Centro de Tecnología Biomédica. CTB. UPM Campus de Montegancedo. Teléfono: +34 91 067 92 50


Coordenadas Geográficas. Grados. * N 40º 24′ 23.76″ * W 3º 50′ 1.32″ Decimales. * N 40.4066 * W 3.8337