Optics, Photonics and Biophotonics Lab

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The optics, photonics and biophotonics group is composed of 6 doctors, 7 PhD  candidates and other students developing their bachelor´s or master´s thesis.  Among the doctors of the group, 4 are professors at the School of Industrial Engineering (ETSII) in the department of applied physics and material Engineering and the rest of them are in their post-doctoral stage.

Our work includes all the value chain, since basic investigation until the development of prototypes. Most of them are focused on the detection of proteins, virus and important biomarkers without the necessity of chemical development, e.  g. Biochip-KIT. Also, we develop a reading method for the biochips, using optical read-out systems very useful for in vitro detection.

Recently, in collaboration with other groups, we are developing systems to immobilize cells with the goal of replace the experimentation in animal systems.

Our group projects are focus in basic investigation and their application in the industrial development with funding of national and international agencies and companies.

Moreover, we participate in projects of science outreach, to promote science vocations in the younger students.

Finally, our group created in 2010 the company BIOD, whose implement and commercialize some the technology developed by the group.

WHO WE ARE

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CONTACT INFORMATION

Group leader: Dr. Miguel Holgado
Phone number: 91 336 46 36 / 38
Mail:  m.holgado@upm.es

Principal Research lines

Photonic transducers for label-free biosensors.
Optical read-out platforms for the development of Point-of-care devices  to present information of clinical interest.
Packaging and KITs focused on multiplexation and user-friendly
Micro-nano fluidics for lab-on-a-chip systems.
Fluidic chips for cells-on-a-chip systems.
Surface functionalization  for bio-receptors and chemical applications.

INFRASTRUCTURE AND TECHNIQUES

The Biophotonics Laboratory is under clean conditions and temperature control, and it is also equipped with clean dry and dust-less air

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MICRO-NANO FABRICATION

• Processing protocols for wet etching and cleaning
• Thin films deposition by spin-coating up to 150 mm wafers,
• Laminar flow class 10 chamber
• Thin films Chemical Vapor deposition CVD (self-produced ) for SiO2 and TiO2
• Thermal processes by hot plates
• Precision Balances
• UV projection lithography mask (resolution up to 500 nm) up to 150 mm wafers
• Nano-imprint lithography (UV-NIL)
• Manufacture by stamping and mold for polymers
• Developing processes for micro-nano lithography
• Fast prototyping manufacture by 3D printer

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OPTICAL CHARACTERIZATION AND READ-OUT SYSTEMS

• Read-out system for continuous flow biomolecular interaction system
• Point of Care systems manufactured by BIOD and designed by the Research group.
• Optical-fiber thin-film metrology (1 nm resolution) UV-VIS for measuring thin film thickness and equipped with camera capable of measuring up to 0.2 mm domains
• FT-VIS-NIR high-resolution spectrometry (pm resolution) and IR spectroscopy in transmission and reflection for VIS-NIR capable of measuring domains at the micrometer domains, also equipped with a microscope.
• Sub-micrometric spatial resolution (up to 600 nm) Reflectometry angle -resolved and ellipsometry valid for thickness measurements on thin films and nanostructures response
analysis
• Optical Microscopy

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BIO-FUNCTIONALIZATION: INMOBILIZATION AND OPTICAL IN-VITRO DETECTION OF BIOMOLECULES

• Assays models: IgG annti-IgG / BSA-anti_BSA
• Infectious diseases: Rotavirus, Dengue, Influenza
• Tear-dry eye monitoring: Tear Biomarkers
• Pathogen detection: Bacteria
• Detection of Allergens and Immunological tolerances

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PUBLICATIONS

Título: Antigen-Antibody affinity for dry eye biomarkers by label free biosensing. Comparison with ELISA technique
Autores: M.F. Laguna, M. Holgado, A. López, B. Santamaría, A. Lavin, J. Soria, T. Suarez, C. Bardina, M. Jara, F.J. Sanza, R. Casquel
Revista: Sensors 2015, 15, 19819-19829; doi:10.3390/s150819819

Título: Arrays of resonant nano-pillars for biochemical sensing
Autores: Ana L. Hernández, R. Casquel, M. Holgado, I. Cornago, F.J. Sanza, B. Santamaría, M. Maigler, F. Fernández, A. Lavín, M.F. Laguna
Revista: Optics Letter, 40 (2015), 2370-2372

Título: Sub-micrometric reflectometry for localized label-free biosensing
Autores: R. Casquel, J.A. Soler, M. Holgado, A. López, A. Lavín, J. de Vicente, F.J. Sanza, M.F. Laguna, M.J. Bañuls, and R. Puchades
Revista: Optics Express, 23 (2015),

Título: Label-free biosensing by means of BICELLs for dry eye
Autores: MFaría Fe Laguna; Francisco J. Sanza; J. Soria; Mónica Jara; Álvaro Lavín;
Rafael Casquel; A. López; T. Suarez; Miguel Holgado
Revista: Sensors and Actuators B 203 (2014) 209–212
Índice de Impacto: 3,980

Título: Silicon nanopillar arrays with SiO2 overlayer for biosensing application
Autores: B. Dev Choudhury; R. Casquel; M.J. Bañuls; F.J. Sanza; M.F. Laguna; M.
Holgado; R. Puchades; A. Maquieira; C.A. Barrios; S. Anand
Revista: Optical Materials Express, 2014
Índice de Impacto: 2,616

Título: Description of an Advantageous Optical Label-Free Biosensing Interferometric Read-Out Method to Measure Biological Species
Autores: Holgado, M.; Sanza, F.J.; López, A.; Lavín, A.; Casquel, R.; Laguna, M.F.
Revista: Sensors 2014, 14, 3675-3689
Índice de Impacto: 2,394

Título: Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method
Autores: Laguna, M.F.; Holgado, M.; Sanza, F.J.; Lavín, A.; López, A.; Casquel, R.
Revista: Sensors 2014, 14, 6695-6700.
Índice de Impacto: 2,394

Título: Development of a versatile biotinylated material based on SU-8
Autores: Francisco J. Ortega, María-Jose ́ Bañuls, Francisco J. Sanza, María F. Laguna, Miguel Holgado, Rafael Casquel, Carlos A. Barrios, David López-Romero, Ángel Maquieira and Rosa Puchades.
Revista: Journal of Material Chemistry B, 1 (2013) 2750-2756
Índice de Impacto: 5,970

Título: Efficient design and optimization of bio-photonic sensing cells (BICELLs) for label free biosensing
Autores: Álvaro Lavín, Rafael Casquel, Francisco J. Sanza, María Fé Laguna, Miguel Holgado.
Revista: Sensors and Actuators B 176 (2013) 753–760
Índice de Impacto: 3,9878. Lugar que ocupa la revista en la categoría: 1 de 26. Tercil: T1

Título: Bio-Photonic Sensing Cells over transparent substrates for anti-gestrinone antibodies biosensing
Autores: F.J. Sanza, M. Holgado, F.J. Ortega, R. Casquel, D. López-Romero, M.F. Laguna, M.J. Bañuls, C.A. Barrios, R. Puchades, A. Maquieira.
Revista: Biosensors and Bioelectronics 26 (2011) 4842–4847
Índice de Impacto: 5,361. Lugar que ocupa la revista en la categoría: 1 de 26. Tercil: T1

Título: Transparent nanometric organic luminescent films as UV active components in photonic structures
Autores: F.J. Aparicio, M. Holgado, I. Blaszczyk-Lezak, A. Borras, A. Griol, C.A. Barrios, R. Casquel, F.J. Sanza, H. Sohlström, M. Antelius, A.R. González-Elipe, A. Barranco.
Revista: Advanced Materials 23 (2011) 761-765
Índice de Impacto: 8,379. Lugar que ocupa la revista en la categoría: 4 de 121. Tercil: T1

Título: UV laser-induced high resolution cleaving of Si wafers for micro-nano devices and polymeric waveguide characterization
Autores: R. Casquel, M. Holgado, J.J. García-Ballesteros, K. Zinoviev, C. Fernández-Sánchez, F.J. Sanza, C. Molpeceres, M.F. Laguna, A. Llobera, J.L. Ocaña, C. Domínguez
Revista: Applied Surface Science 257 (2011) 5424 – 54248
Índice de Impacto: 2.103. Lugar que ocupa la revista en la categoría: 6 de 17. Tercil: T2

Título: Cost-effective SU-8 micro-structures by DUV excimer laser lithography for label-free biosensing
Autores: F.J. Sanza, R. Casquel, M.F. Laguna, M. Holgado, C.A. Barrios, F.J. Ortega M.J. Bañuls, D. López-Romero, R. Puchades, A. Maquieira
Revista: Applied Surface Science 257 (2011) 5403 – 5407
Índice de Impacto: 2.103. Lugar que ocupa la revista en la categoría: 6 de 17. Tercil: T2

Título: Study of the refractive index change in a-Si:H thin films patterned by 532 nm laser radiation for photovoltaic applications
Autores: M. Colina, C. Molpeceres, M. Holgado, J. Gandia, O. Nos, J.L. Ocaña,
Revista: Thin Solid Film 518 (2010) 5331- 5339
Índice de Impacto: 1,727. Lugar que ocupa la revista en la categoría: 4 de 17. Tercil: T1

Título: Label-free biosensing by means of periodic lattices of high aspect ratio SU8 nano-pillarss
Autores: M. Holgado, C.A. Barrios, F.J. Ortega, F.J. Sanza, R. Casquel, M.F. Laguna, M.J. Bañuls, D. López-Romero, R. Puchades, A. Maquieira
Revista: Biosensors and Bioelectronics 25 (2010) 2553–2558
Índice de Impacto: 5,429. Lugar que ocupa la revista en la categoría: 2 de 70. Tercil: T1

Título: High aspect-ratio SU-8 resist nano-pillar lattice by e-beam direct writing and its application for liquid trapping
Autores: David Lopez-Romero; Carlos Angulo Barrios, Miguel Holgado, Maria Fe Laguna, Rafael Casquel
Revista: Microelectronic Engineering 87 (2010) 663–667
Índice de Impacto: 1,488. Lugar que ocupa la revista en la categoría: 74 de 246. Tercil: T1

Título: Analysis of plasma thermal surface effects on the residual stress field induced by LSP in Al2024-t351
Autores: M. Morales, J. A. Porro, C. Molpeceres, M. Holgado, J. L. Ocaña
Revista: Journal of optoelectronics and advanced materials 12 (2010) 718-722
Índice de Impacto: 0,433. Lugar que ocupa la revista en la categoría: 175 de 214. Tercil: T3

Título: Simultaneous reflectivity, ellipsometry and spectrometry measurements in submicron structures for Liquid sensing
Autores: M. Holgado, R. Casquel, M. Morales, C. Molpeceres, J.L. Ocaña
Revista: Sensor Letters 6 (2008) 564-569
Índice de Impacto: 1,160. Lugar que ocupa la revista en la categoría: 24 de 56. Tercil: T2

Título: Reconfiguration of microring resonators by liquid adhesion
Autores: C.A. Barrios, M. Holgado, O. Guarneros, K.B. Gylfason,3 B. Sánchez, R. Casquel and H. Sohlström
Revista: Applied Physics Letters 93 (2008) 203114-203117
Índice de Impacto: 3,726. Lugar que ocupa la revista en la categoría: 10 de 95. Tercil: T1

Título: Label-free optical biosensing with slot-waveguides
Autores: Carlos A. Barrios, María José Bañuls, Victoria González-Pedro, Kristinn B. Gylfason, Benito Sánchez, Amadeu Griol, A. Maquieira, H. Sohlström, M. Holgado, R. Casquel
Revista: Optics Letters 33 (2008) 708-710
Índice de Impacto: 3,772. Lugar que ocupa la revista en la categoría: 4 de 64. Tercil: T1

Título: Slot-waveguide biochemical sensor
Autores: Carlos A. Barrios, Kristinn B. Gylfason, B. Sánchez, Amadeu Griol, H. Sohlstrom, M. Holgado, R. Casquel
Revista: Optics Letters 32 (2007) 3080-3082
Índice de Impacto: 3,711. Lugar que ocupa la revista en la categoría: 1 de 64. Tercil: T1

Título: Optical characterization of extremely small volumes of liquid in sub-micro-holes by simultaneous reflectivity, ellipsometry and spectrometry
Autores: M. Holgado, R. Casquel, M. Morales, C. Molpeceres, J.L. Ocaña
Revista: Optics Express 15 (2007) 13318-13329
Índice de Impacto: 3,709. Lugar que ocupa la revista en la categoría: 2 de 64. Tercil: T1

Título: Demonstration of Slot-waveguide structures on silicon nitride / silicon platform
Autores: Carlos A. Barrios, B. Sánchez, K. B. Gylfason, A. Griol, H. Sohlstrom, M. Holgado, R. Casquel
Revista: Optics Express 15 (2007) 6846-6856
Índice de Impacto: 3,709. Lugar que ocupa la revista en la categoría: 2 de 64. Tercil: T1

Title: Development towards compact nitrocellulose-based interferometric biochip for Dry Eye MMP9 label-free in-situ diagnosis.
Authors: Beatriz Santamaría, María F. Laguna, David López-Romero, Ana L. Hernández, Francisco J. Sanza, Álvaro Lavín, Rafael Casquel , María V. Maigler, Rocio L. Espinoza, Miguel Holgado.
Journal: Sensors 2017, 17, x

Title: Optical sensor based on periodic array of resonant nanopillars for real time monitoring
Authors: Fátima Fernádez, Paula Ciaurriz, Iñaki Cornago, Ana L. Hernández, F.J. Sanza, M.F. Laguna, M. Holgado.
Journal: Sensors and Actuators B 244 (2017) 323-326
DOI: http://dx.doi.org/10.1016/j.snb.2016.12.140

Title: Resonant nanopillars arrays for label-free biosensing.
Authors: Ana L. Hernández, Rafael Casquel, Miguel Holgado, Iñaki Cornago, Fátima Fernández, Paula Ciaurriz, Francisco J. Sanza, Beatriz Santamaría, María V. Maigler, María Fe Laguna.
Journal: Optics Letters Vol 41, No 23 (2016).
DOI: http://dx.doi.org/10.1364/OL.41.005430

Title: Towards reliable optical label-free point-of-care (PoC) biosensing devices.
Authors: M. Holgado, M.V. Maigler, B. Santamaria, A.L. Hernández, A. Lavín, M.F. Laguna, F.J. Sanza, D. Granados, R. Casquel, J. Portilla, T. Riesgo.
Journal: Sensors and Actuators B 236 (2016) 765-772
DOI: http://dx.doi.org/10.1016/j.snb.2016.06.047

Title: Direct Laser Interference Patterning (DLIP) technique applied to the development of optical biosensors based on Biophotonic Sensing Cells (BICELLs).
Authors: F.J. Sanza, D. Langheinrich, J. Berger, A.L. Hernandez, S. Dani, R. Casquel, A. Lavín, A. Otón, B. Santamaría, M. Laguna, A.F. Lasagni, M. Holgado.
Journal: SPIE Vol. 9351, 935114 (2015)
DOI: 10.1117/12.2077292

Title: Bulk sensing performance comparison between silicon dioxide and resonant high aspect ratio nanopillars arrays fabricated by means of interference lithography.
Authors: I. Cornago, A.L. Hernández, R. Casque, M.holgado, M.F. Laguna, F.J. Sanza, J. Bravo.
Journal: Optical Material express (2016), Vol. 6, No. 7
DOI:10.1364/OME.6.002264

Title: Performance evaluation for different sensing surface of BICELLs biotransducers for dry eye biomarkers.
Authors: M.F. Laguna, M. Holgado, B. Santamaria, A. López, M. Maigler, A. Lavin, J. de Vicente, J. Soria, T. Suárez, C. Bardina, M. Jara, F.J. Sanza, R. Casquel, A. Oton, T. Riesgo.
Journal: SPIE Vol. 9337, 93370J (2015)
DOI: 10.1117/12.2077174

Title: Sensitive metal layer-assisted guided-mode resonance SU8 nanopillar array for label-free optical biosensing.
Authors: V. Canalejas-Tejero, A. López, R. Casque, M. Holgado, C.A. Barrios.
Journal: Sensor and Actuators B: Chemical (2005).
DOI: http://dx.doi.org/10.1016/j.snb.2015.11.114

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